Translational_Unit

Part:BBa_K415116:Design

Designed by: Grant Robinson   Group: iGEM10_MIT   (2010-07-06)


RBS : pIX


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This ~300 bp part was produced via PCR from the genome of bacteriophage M13X07. The primers read as follows:

Forward: [To be specified] Reverse: [To be specified]

These primers ensured that the PCR included the standard BioBrick restriction sites.


Source

This part originates in bacteriophage M13X07, specifically the 99 base-pair sequence for gIX and a weak ribosome binding site [To be specified].

References